Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal.
نویسندگان
چکیده
Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).
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عنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 26 6 شماره
صفحات -
تاریخ انتشار 2010